Bacteriophage-resistance mechanisms examined by transfection ofLactococcus lactissubsp.lactis4513-5 mediated by high voltage electroporation
✍ Scribed by Katja M. Meyer; Roland Süssmuth
- Book ID
- 104652577
- Publisher
- Springer
- Year
- 1992
- Tongue
- English
- Weight
- 655 KB
- Volume
- 37
- Category
- Article
- ISSN
- 1432-0614
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✦ Synopsis
Phage adsorption tests and transfection by electroporation were carried out to decide whether phage-resistance in Lactococcus lactis subsp, lactis strain 4513-5 is based on intracellular or extracellular mechanisms. Using high voltage (12.5 kV/cm) electropotation, untreated phage DNA was introduced into phage-sensitive and phage-resistant cells. Since phages showed low adsorption frequencies on resistant bacteria, resistance is localized in the cell wall preventing phage DNA from entering the cell. This is the only mechanism responsible for the resistance of L. lactis subsp, lactis 4513-5 against its homologous phage P4513-K12 and non-homologous phages P05M-13 and P05M-47, but not against phage P530-7 and phage P530-12. In the case of the latter two phage strains, intracellular resistance mechanisms are involved and discussed.