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Bacteriophage P4 DNA replication: Nucleotide sequence of the P4 replication gene and the cis replication region

✍ Scribed by John Flensburg; Richard Calendar

Book ID
118918715
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
781 KB
Volume
195
Category
Article
ISSN
0022-2836

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A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/