Cloning of the replication gene P of bac
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Klinkert, Jörg ;Klein, Albrecht
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Article
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1979
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Springer
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English
⚖ 844 KB
A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/