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Bacteriophage P2-lambda interference: II. Effects on the host under the control of lambda genes O and P

✍ Scribed by François Brégégère


Book ID
118917866
Publisher
Elsevier Science
Year
1976
Tongue
English
Weight
622 KB
Volume
104
Category
Article
ISSN
0022-2836

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Cloning of the replication gene P of bac
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A restriction fragment of lambdaDNA carrying the P gene was cloned in the high copy number plasmid RSF2124. Cells harbouring this new plasmid RSF2124/lambdaE complement lambdaPam80 phage. A lac promoter-operator region (lacP), produced by EcoRI digestion of plasmid pKB252, was inserted into RSF2124/