Bacteriophage-associated lyase activity against Klebsiella serotype K64 capsular polysaccharide

✍ Scribed by Neil Ravenscroft; Alistair M. Stephen; Edwin H. Merrifield

• Book ID: 102992380
• Publisher: Elsevier Science
• Year: 1987
• Tongue: English
• Weight: 689 KB
• Volume: 167
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(87)80283-5

✦ Synopsis

Bacteriophage 464 possesses a lyase that depolymerises the capsular polysaccharide of Klebsiella K64 into a hexasaccharide having an unsaturated derivative of glucuronic acid at the non-reducing end (1). The unsaturated hex-4-enuronic acid residue generated was characterised spectroscopically (u.v. and n.m.r.) and by g.l.c.-m.s. after hydrogenation of the double bond. Partial hydrolysis, Smith degradation, methylation analysis, and n.m.r. spectroscopy have been used to establish the structures of oligosaccharides produced from the polysaccharide. Evidence from 'H-n.m.r. spectroscopy indicates that the D-Manp residue that undergoes fission is /3.