Bacteriology MD0856

✍ Scribed by US Army medical department

• Year: 2006
• Tongue: English
• Leaves: 265
• Series: US Army medical course
• Edition: 100
• Category: Library

✦ Table of Contents

DEVELOPMENT
ADMINISTRATION
TABLE OF CONTENTS
INTRODUCTION
LESSON 1
Section I. HOST-PARASITE RELATIONSHIPS
1-1. CAUSATION OF MICROBIAL DISEASE
1-2. TERMINOLOGY
1-3. THE INFECTIOUS PROCESS
Section II. ASPECTS OF PATHOGENICITY
1-4. CAPSULATION
1-5. BACTERIAL TOXINS
1-6. EXTRACELLULAR ENZYMES
Section III. HOST RESISTANCE
1-7. NONSPECIFIC FACTORS
1-8. SPECIFIC FACTORS-IMMUNOLOGICAL RESPONSE
Section IV. THE BACTERIAL CELL
1-9. AUTOTROPHIC BACTERIA
1-10. HETEROTROPHIC BACTERIA
1-11. MORPHOLOGY
1-12. STRUCTURE OF BACTERIA
1-13. IDENTIFICATION OF BACTERIA
1-14. PATHOGENIC BACTERIA
Section V. COLLECTING AND PROCESSING OF BACTERIOLOGICAL
1-15. BASIC PRINCIPLES
1-16. COLLECTING AND PROCESSING OF BLOOD CULTURES.
1-17. COLLECTING AND PROCESSING OF SPECIMENS FROM THE RESPIRATORY TRACK
1-18. COLLECTlNG AND PROCESSING URINE SPECIMENS
1-19. COLLECTING AND PROCESSING OF FECAL SPECIMENS
1-20. COLLECTING AND PROCESSING BODY FLUIDS
1-21. COLLECTING AND PROCESSING EXUDATE SPECIMENS
1-22. PROCESSING OF SPECIMENS FOR SHIPMENT
EXERCISES
SOLUTIONS
LESSON 2
Section I. MICROSCOPIC EXAMINATION OF BACTERIA
2-1. GENERAL COMMENTS
2-2. PREPARATION OF SMEARS FOR STAINING
2-3. ACID-FAST STAIN (ZIEHL-NEELSEN)
2-4. ACID-FAST STAIN (KINYOUN MODIFIED)
2-5. CAPSULE OUTLINE (INDIA INK)
2-6. CAPSULE STAIN (HISS METHOD)
2-7. FLAGELLA STAIN (LEIFSON METHOD MODIFIED)
2-8. GRAM STAIN (HUCKER'S MODIFICATION)
2-9. METHYLENE BLUE STAIN
2-10. SPORE STAIN (WIRTZ-CONKLIN)
2-11. STAINING OF BLOOD SMEARS
2-12. PREPARATION OF WET UNSTAINED MOUNTS FOR MICROSCOPIC EXAMINATION
Section II. CULTIVATION OF BACTERIA (MEDIA)
2-13. INTRODUCTION
2-14. COMPOSITION OF MEDIA
2-15. HYDROGEN ION CONCENTRATION (pH)
Section III. ENVIRONMENTAL FACTORS
2-16. TEMPERATURE
2-17. OXYGEN REQUIREMENTS
2-18. CARBON DIOXIDE REQUIREMENTS
Section IV. ANAEROBIC METHODS
2-19. BASIC PRINCIPLES
2-20. COLLECTION AND TRANSPORT OF CLINICAL MATERIALS
2-21. EXAMINATION OF SMEARS
2-22. INOCULATION OF PRIMARY ISOLATION MEDIA
2-23. INCUBATION AND SUBCULTURE
2-24. PROCEDURE AFTER INCUBATION
Section V. ANTIBACTERIAL AGENTS, STERILIZATION, AND ASEPTIC TECHNIQUE
2-25. ANTIBACTERIAL AGENTS
2-26. GENERAL COMMENTS ON STERILIZATION
2-27. MEDIA PREPARATION
2-28. HEAT
2-29. FILTRATION
2-30. RADIATION
2-31. GENERAL COMMENTS ON ASEPTIC TECHNIQUE
2-32. WORKING AREA
2-33. DISPOSAL OF CONTAMINATED MATERIALS
2-34. PERSONAL CLEANLINESS
Section VI. ISOLATION OF BACTERIA
2-35. INTRODUCTION
2-36. INOCULATING LOOPS AND NEEDLES
2-37. STREAK PLATE
2-38. POUR PLATE METHOD
2-39. TEST TUBE CULTURES
EXERCISES
SOLUTIONS
LESSON 3
Section I. COMMON TESTS
3-1. BILE SOLUBILITY TEST
3-2. CARBOHYDRATE FERMENTATION TEST
3-3. CATALASE TEST
3-4. CITRATE TEST
3-5. COAGULASE TEST
3-6. FERRIC CHLORIDE TEST
3-7. GELATIN LIQUEFACTION TEST
3-8. HYDROGEN SULFIDE TEST (LEAD ACETATE PAPER TEST)
3-9. INDOLE TEST
3-10. METHYL RED TEST
3-11. NITRATE REDUCTION TEST
3-12. OXIDASE TEST
3-13. UREASE TEST
3-14. VOGES-PROSKAUER TEST
Section II. GRAM-POSITIVE COCCI: STAPHYLOCOCCI
3-15. GENERAL COMMENTS ABOUT GRAM-POSITIVE COCCI
3-16. PATHOGENICITY OF STAPHYLOCOCCI
3-17. CULTURAL CHARACTERISTICS OF STAPHYLOCOCCI
3-18. LABORATORY INDENTIFICATION OF STAPHYLOCOCCI
3-19. CONFIRMATORY PROCEDURES
Section III. GRAM-POSITIVE COCCI: STREPTOCOCCI
3-20. INTRODUCTION TO THE STREPTOCOCCI
3-21. HEMOLYTIC PATTERNS
3-22. PATHOGENICITY OF THE STREPTOCOCCI
3-23. CULTURAL CHARACTERISTICS OF THE STREPTOCOCCI
3-24. GENERAL IDENTIFICATION STREPTOCOCCI
3-25. BETA HEMOLYTIC STREPTOCOCCI
3-26. DIFFERENTIATION OF OTHER STREPTOCOCCI
Section IV. GRAM-POSITIVE COCCI: PNEUMOCOCCI
3-27. MORPHOLOGY OF PNEUMOCOCCI
3-28. GROWTH REQUIREMENTS OF PNEUMOCOCCI
3-29. COLONY CHARACTERISTICS OF PNEUMOCOCCI
3-30. PATHOGENICITY OF PNEUMOCOCCI
3-31. LABORATORY IDENTIFICATION OF PNEUMOCOCCI
3-32. CONFIRMATORY STUDIES
EXERCISES
SOLUTIONS
LESSON 4
Section I. INTRODUCTION
4-1. GENERAL COMMENTS ABOUT THE NEISSERIAE
4-2. COMMON CHARACTERISTICS OF THE NEISSERIAE
Section II. NEISSERIA GONORRHOEAE
4-3. PATHEGENICITY
4-4. CRITERIA FOR WOMEN
4-5. CRITERIA FOR MEN
4-6. SPECIAL SITUATIONS
4-7. OBTAINING SPECIMENS FOR CULTURE--WOMEN
4-8. OBTAINING SPECIMENS FOR CULTURE--MEN
4-9. CONDITIONS FOR INOCULATION OF MODIFIED THAYER-MARTIN MEDIUM
4-10. MODIFIED THAYER-MARTIN MEDIUM IN PLATES
4-11. CO2-GENERATING TABLETS
4-12. MODIFIED THAYER-MARTIN MEDIUM BOTTLES
4-13. INCUBATION
4-14. PRESUMPTIVE IDENTIFICATION OF NEISSERIA GONORRHOEAE
4-15. CONFIRMATORY IDENTIFICATION OF N. GONORRHOEAE
4-16. ARBOHYDRATE UTILIZATION TESTS FOR NEISSERIA GONORRHOEAE
4-17. CONFIRMATION OF NEISSERIA GONORRHOEAE
Section III. NEISSERIA MENINGITIDIS
4-18. PATHOGENICITY
4-19. SIMILARITY TO GONOCOCCI
4-20. SOURCE
4-21. IDENTIFICATION
Section IV. GRAM-POSITIVE BACILLI: CORYNEBACTERIA AND RELATEDSPECIES
4-22. GENERAL COMMENTS ABOUT THE GRAM-POSITIVE BACILLI
4-23. GENERAL COMMENTS ABOUT THE GENUS CORYNEBACTERIUM
4-24. CULTIVATION OF CORYNEBACTERIA
4-25. PATHOGENICITY OF CORYNEBACTERIA
4-26. LABORATORY IDENTIFICATION OF CORYNEBACTERIA
4-27. LISTERIA MONOCYTOGENES
4-28. ERYSIPELOTHRIX RHUSIOPATHIAE
Section V. GRAM-POSITIVE BACILLI: BACILLUS SPECIES
4-29. GENERAL COMMENTS ABOUT THE GENUS BACILLUS
4-30. BACILLUS SPORE FORMATION
4-31. BACILLUS CULTURES
4-32. PATHOGENICITY OF BACILLUS
4-33. LABORATORY IDENTIFICATION OF BACI-SPECIES
Section VI. GRAM-POSITIVE BACILLI: CLOSTRIDIA
4-34. GENERAL COMMENTS ABOUT THE GENUS CLOSTRIDIUM
4-35. GROWTH REQUIREMENT OF CLOSTRIDIA
4-36. APPEARANCE OF CLOSTRIDIA
4-37. PATHOGENICITY OF CLOSTRIDIA
4-38. LABARORATORY IDENTIFICATION OF CLOSTRIDIUM SPECIES
Section VII. GRAM-POSITIVE BACILLI: MYCOBACTERIA
4-39. GENERAL COMMENTS ABOUT THE GENUS MYCOBACTERIUM
4-40. PATHOGENICITY
4-41. GENERAL PROCEDURES
4-42. APPEARANCE OF MYCOBACTEIRA
4-43. TRATION FOR CULTIVATION
4-44. CULTIVATION OF MYCOBACTERIA
4-45. VIRULENT STRAINS OF MYCOBACTERIA
4-46. SAPROPHYTIC MYCOBACTERIA
4-47. CONFIRMATORY PROCEDUES FOR MYCOBACTERIA
4-48. CATALASE TEST (FOR ACID-FAST BACILLI)
4-49. NEUTRAL RED TEST (FOR ACID-FAST BACILLI)
4-50. NIACIN TEST (FOR ACID-FAST BACILLI)
EXERCISES
SOLUTIONS
LESSON 5
Section I. INTRODUCTION
5-1. DEFINITION
5-2. NORMAL FLORA AND GENERAL IDENTIFICATION OF SPECIES
5-3. MORPHOLOGY AND CULTURE
Section II. PATHOGENICITY OF ENTEROBACTERIACEAE
5-4. PATHOGENICITY OF THE GENUS ESCHERICHIA
5-5. PATHOGENICITY OF THE GENUS KLEBSIELLA
5-6. PATHOGENICITY OF THE GENUS ENTEROBACTER
5-7. PATHOGENICITY OF THE GENUS PROTEUS
5-8. PATHOGENICITY OF THE GENUS SALMONELLA
5-9. PATHOGENICITY OF THE GENUS SHIGELLA
Section III. ENTERIC MEDIA
5-10. CATEGORIES OF ENTERIC MEDIA
5-11. LACTOSE FERMENTATION
5-12. DIFFERENTIAL MEDIA
5-13. SELECTIVE MEDIA
5-14. ENRICHED MEDIA
5-15. TUBED DIFFERENTIAL MEDIA
5-16. ISOLATION OF ENTERIC PATHOGENS FROM FECAL SPECIMENS
Section IV. PRELIMINARY SCREENING OF CULTURES FOR ENTEROBACTERIACEAE
5-17. COLONY CHARACTERISTICS
5-18, USE OF LESS INHIBITORY MEDIA
5-19. INDOLE, METHYL RED, VOGES-PROSKAUER, and CITRATE TESTS
Section V. IDENTIFICATION OF ENTERIC ORGANISMS
5-20. GENERAL
5-21. LYSINE DECARBOXYLASE TEST (FALKOW METHOD)
5-22. DECARBOXYLASE TESTS (MOELLER METHOD)
5-23. PHENYLALANINE DEAMINASE TEST
5-24. MALONATE TEST
5-25. PECTOLYSIS TEST (MARTIN AND EWING)
5-26. MOTILITY TEST
5-27. THE PROTEUS AND PROVIDENCIA GENERA
5-28. THE ENTEROBACTER AND KLEBSIELLA GENERA
5-29. THE GENUS SALMONELLA
5-30. THE GENUS SHIGELLA
5-31. THE GENUS ESCHERICHIA
5-32. MORPHOLOGY
5-33. CULTURAL CHARACTERISTICS
5-34. LABORATORY IDENTIFICATION
5-35. PATHOGENICITY
EXERCISES
SOLUTIONS
LESSON 6
LESSON ASSIGNMENT
Section I. PSEUDOMONAS
6-1. INTRODUCTION
6-2. PSEUDOMONAS AERUGINOSA
6-3. PSEUDOMONAS PSEUDOMALLEI
Section II. BRUCELLA
6-4. GENERAL TYPES
6-5. CULTURE
6-6. PATHOGENICITY
6-7. LABORATORY IDENTIFICATION
6-8. MORPHOLOGY
6-9. CULTURAL CHARACTERISTICS
6-10. PATHOGENICITY AND IDENTIFICATION
Section IV. BORDETELLA
6-11. INTRODUCTION
6-12. IDENTIFICATION
Section V. FRANCISELLA TULARENSIS
6-13. MORPHOLOGY
6-14. CULTURAL CHARACTERI.STICS
6-15. LABORATORY IDENTIFICATION
6-16. PATHOGENICITY
Section VI. MISCELLANEOUS ORGANISMS
6-17. BACTEROIDES FRAGILIS
6-18. STREPTOBACILLUS MONILIFORMIS
Section VII. ANTIBIOTIC SENSITIVITY TESTS
6-19. GENERAL COMMENTS
6-20. ANTIBIOTIC RESISTANCE
6-21. PREVENTING EMERGENCE OF RESISTANT STRAINS OF BACTERIA
6-22. LABORATORY ANTIBIOTICS METHODS FOR DETERMINING SENSITIVITY OF AN ORGANISM TO ANTIBOTICS
6-23. KIRBY-BAUER METHOD FOR DETERMINING BACTERIAL SENSITIVITY
EXERCISES
SOLUTIONS