Expression of functional Candida antarct
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Chang-Gil Park; Tae-Wan Kim; In-Seok Oh; Jae Kwang Song; Dong-Myung Kim
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Article
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2009
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American Institute of Chemical Engineers
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English
⚖ 161 KB
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## Abstract This article reports the cell‐free expression of functional Lipase B from __Candida antarctica__ (CalB) in an __Escherichia coli__ extract. Although most of the cell‐free synthesized CalB was insoluble under conventional reaction conditions, the combined use of molecular chaperones led