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Bacterial formate dehydrogenase. Increasing the enzyme thermal stability by hydrophobization of alpha-helices

✍ Scribed by Alexandra M. Rojkova; Andrey G. Galkin; Ludmila B. Kulakova; Alexander E. Serov; Pavel A. Savitsky; Vladimir V. Fedorchuk; Vladimir I. Tishkov


Book ID
117110458
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
755 KB
Volume
445
Category
Article
ISSN
0014-5793

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Serial increase in the thermal stability
✍ Satoshi Akanuma; Akihiko Yamagishi; Tairo Oshima; Nobuo Tanaka πŸ“‚ Article πŸ“… 1998 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 856 KB

## Abstract We improved the thermal stability of 3‐isopropylmalate dehydrogenase from __Bacillus subtilis__ by an in vivo evolutionary technique using an extreme thermophile, __Thermus thermophilus__, as a host cell. The __leuB__ gene encoding B. __subtilis__ 3‐isopropylmalate dehydrogenase was int