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Bacterial expression systems for recombinant protein production: E. coli and beyond

✍ Scribed by Rachel Chen


Book ID
119200082
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
218 KB
Volume
30
Category
Article
ISSN
0734-9750

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A genetic strategy to enhance recombinant protein production is discussed. A small DNA bending protein, Fis, which has been shown to activate rRNA synthesis upon a nutrient upshift, was overexpressed in E. coli strain W3110 carrying vector pUCR1. Overexpres- sion of Fis during exponential growth was