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Bacterial expression and characterization of starfish phospholipase A2

โœ Scribed by Hideki Kishimura; Takao Ojima; Kenji Hayashi; Kiyoyoshi Nishita


Book ID
117733326
Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
601 KB
Volume
128
Category
Article
ISSN
1096-4959

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A gene coding for the (pr0)phospholipase A, (PLA2) from bovine pancreas has been designed, synthesized, and expressed in Escherichia coli. The gene was designed with a variety of restriction sites that will facilitate future mutagenesis studies. Ccdons occurring frequently in prokaryotic systems wer