Bacterial community changes in diesel-oil-contaminated soil microcosms biostimulated with Luria–Bertani medium or bioaugmented with a petroleum-degrading bacterium, Pseudomonas aeruginosa strain WatG
✍ Scribed by Akio Ueno; Yukiya Ito; Yusuke Yamamoto; Isao Yumoto; Hidetoshi Okuyama
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 233 KB
- Volume
- 46
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Abstract
The microbial community structure of diesel‐oil‐contaminated soil microcosms biostimulated with Luria–Bertani medium (LB‐BS) or bioaugmented with a petroleum‐degrading bacterium, Pseudomonas aeruginosa strain WatG (WatG‐BA), was investigated by denaturing gradient gel electrophoresis (DGGE) and by monitoring diesel oil degradation. The degradation in WatG‐BA (64.0% ± 4.2%) was higher than that in LB‐BS (49.5% ± 12.0%) during the first two weeks. The microbial community in WatG‐BA, which was markedly dominated by strain WatG, was much simpler than that in LB‐BS, where hydrocarbon degraders occurred after a lag of 3–7 days after the addition of diesel oil. The clustering profiles of the DGGE banding patterns of the two soil microcosms were only 12% similar. This difference is probably due to antibacterial substances, such as rhamnolipids, secreted by strain WatG. (© 2006 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)