A method is described whereby bacterial mutants (sun) may be selected which are able to specifically suppress mutations in the N gene of bacteriophage lambda. The sun mutations seem to be allelic to suA mutations, which suppress the polarity of nonsense codons, since suA mutants have all of the prop
✦ LIBER ✦
Bacteria with defective rho factors suppress the effects ofN mutations in bacteriophage λ
✍ Scribed by Richardson, John P. ;Fink, Pamela ;Blanchard, Kerry ;Macy, Marthy
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 508 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0026-8925
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We analysed the effects on the expression of the gal operon of six phenotypically different mutations in the Escherichia coli RNA polymerase genes in combination with wild-type, rho, and mutant, rho15, alleles of the gene for the transcription termination factor. RNA polymerase mutations can enhance