Backbone NMR assignments of a topologically knotted protein in urea-denatured state
✍ Scribed by Shu-Ju Micky Hsieh, Anna L. Mallam, Sophie E. Jackson…
- Book ID
- 120903212
- Publisher
- Springer-Verlag
- Year
- 2013
- Tongue
- English
- Weight
- 364 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1874-2718
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pSRII, pH 5.9, 60 mm DHPC (non-deuterated). Maximum entropy data reconstruction was used in F2 and F3. 15 N relaxation data were measured at 14.1 T on a 2 H, 15 N-labeled sample using TROSYmodified sequences. 3D 15 N NOESY experiments were recorded as HMQC and TROSY versions.
## Abstract __Streptomyces__ subtilisin inhibitor (SSI) is known to exist in at least two distinct denatured states, cold‐denatured (D') and heat‐denatured (D) under acidic conditions. In the present work, we investigated the manner how increasing urea concentration from 0 to 8 M changes the polype