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Backbone NMR assignment of the 29.6

✍ Scribed by Mariana Gallo; Sonia Melino; Riccardo Melis; Maurizio Paci; Daniel O. Cicero


Publisher
Springer Netherlands
Year
2006
Tongue
English
Weight
162 KB
Volume
36
Category
Article
ISSN
0925-2738

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The conventional HNCA pulse sequence suffers from the ambiguity that it cannot distinguish inter-and intraresidue correlations because the one-bond and two-bond J(NC Ξ± ) coupling constants are of similar magnitude. This paper presents a novel pulse sequence, sequential HNCA, that leads to a spectrum