Backbone and Side-Chain 13C and 15N Signal Assignments of the α-Spectrin SH3 Domain by Magic Angle Spinning Solid-State NMR at 17.6 Tesla

Assignment of the Nonexchanging Protons

Assignment of the Nonexchanging Protons of the α-Spectrin SH3 Domain by Two- and Three- Dimensional 1H–13C Solid-State Magic-Angle Spinning NMR and Comparison of Solution and Solid-State Proton Chemical Shifts

✍ Barth-Jan van Rossum; Federica Castellani; Kristina Rehbein; Jutta Pauli; Hartmu 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 215 KB 👁 1 views

The assignment of nonexchanging protons of a small microcrystalline protein, the alpha-spectrin SH3 domain (7.2 kDa, 62 residues), was achieved by means of three-dimensional (3D) heteronuclear (1H-13C-13C) magic-angle spinning (MAS) NMR dipolar correlation spectroscopy. With the favorable combinatio