✦ LIBER ✦
Azeotropic phenomena during separation of dilute mixtures of proteins by simulated moving bed chromatography
✍ Scribed by Joukje Houwing; Hugo AH Billiet; Hans A Wesselingh; Luuk AM van der Wielen
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1999
- Tongue
- English
- Weight
- 91 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0268-2575
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✦ Synopsis
Recently, we have introduced the use of a salt gradient over a simulated moving bed system used for puriücation of proteins using ion exchange chromatography. This paper describes azeotropic phenomena in such a system, where complete separation of mixtures of proteins is not possible at certain salt concentrations, due to a reversing selectivity. These phenomena impose additional restrictions on the selection of the ýow rates in each of the sections of the simulated moving bed which are discussed.