Azaguanine resistant hamster cell lines not deficient in hypoxanthine-guanine phosphoribosyl transferase

✍ Scribed by John Morrow; Joseph Colofiore; David Rintoul

• Publisher: John Wiley and Sons
• Year: 1973
• Tongue: English
• Weight: 279 KB
• Volume: 81
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1040810112

✦ Synopsis

Abstract

Using a serial selection technique in which Chinese hamster cells were treated first with 8‐azaguanine and then subsequently with HAT medium it was found that approximately 15% of azaguanine resistant clones were also resistant to HAT. Several such clones were subcultured and found to be stably resistant to azaguanine, in some cases at a higher level than the usual azaguanine resistant mutants which are HAT sensitive. Measurements of hypoxanthine‐guanine phosphoribosyl transferase levels were in some cases lower than the parental line but in three of the clonal lines were higher than the parental strain. The fact that azaguanine resistant lines constitute a biochemically heterogeneous population underscores the importance of careful characterization of mammalian cell culture variants.