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Axotomy induces preprotachykinin gene expression in a subpopulation of dorsal root ganglion neurons

✍ Scribed by K. Noguchi; R. Dubner; M. De Leon; E. Senba; M. A. Ruda

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 891 KB
Volume: 37
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490370506

No coin nor oath required. For personal study only.

✦ Synopsis

The distribution of dorsal root ganglion (DRG) cell sizes that show changes in preprotachykinin (PPT) gene expression and substance P (SP) levels following axotomy was examined using RNA blot analysis, in situ hybridization histochemistry, and immunocytochemistry. PPT mRNA was induced in medium-sized (1,000-2,000 pm') and large-sized (>2,000 pm') cells in the DRG after axotomy. There was a 165% increase in the number of labeled cells after sciatic transection and a 260% increase after spinal nerve transection which results in axotomy of all the cells in the ganglion. The further increase after spinal nerve transection suggests that the induction occurred in axotomized neurons. PPT mRNA label was also present in a reduced number of small (< 1,000 pm2) cells after axotomy. SP immunoreactivity was also induced in medium-and large-sized cells and reduced in small-sized cells. Our findings suggest that the expression of the PPT gene and SP is differentially regulated in different subpopulations of DRG neurons after axotomy and is consistent with the hypothesis that tachykinins may be important in both sensory transmission and regeneration.

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