Axillary shoot proliferation and growth ofPopulus deltoidesshoot cultures
โ Scribed by Gary D. Coleman; Stephen G. Ernst
- Book ID
- 104674857
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 587 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0721-7714
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โฆ Synopsis
Shoot cultures of four genotypes of Populus deltoides Bartr. ex Marsh. were established from adventitious shoots regenerated from internodal stem explants. Stable shoot cultures for all four genotypes were maintained in a continuous culture regime for over one year. The stable shoot cultures were used as explants to investigate the effects of zeatin concentration and genotype on axillary shoot production and growth. The concentration of zeatin significantly affected the production of axillary shoots, with 1.0 mgL(-1) zeatin producing the greatest number of shoots (31.0 shoots per culture vessel) while 0.25 mgL(-1) zeatin produced the greatest growth (5.9 mg per axillary shoot) when measured by dry weight accumulation per shoot. Genotypic differences were significant in the production and growth of axillary shoots.
๐ SIMILAR VOLUMES
The in vitro shoot proliferation ofPopulus alba XP. grandidentata was affected by the medium consistency and shoot density, but not by three sizes of vessels. After 4 weeks of culture, the fresh weight and number of shoots per explant on liquid medium were significantly greater than those on agar-so