Recombinant protein production in Escherichia coli can be significantly reduced by acetate accumula- tion. It is demonstrated that acetate production can be detected on-line with a standard dissolved oxygen sensor by superimposing short pulses to the substrate feed rate. Assuming that acetate format
✦ LIBER ✦
Avoiding acetate accumulation in Escherichia coli cultures using feedback control of glucose feeding
✍ Scribed by M. Åkesson; P. Hagander; J. P. Axelsson
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 190 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0006-3592
- DOI
- 10.1002/bit.1054
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