Avidin as a precipitant for biotin-labeled antibody in a radioimmunoassay for carcinoembryonic antigen

✍ Scribed by Brian R. Clark; Charles W. Todd

• Book ID: 102628363
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 437 KB
• Volume: 121
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90477-8

✦ Synopsis

Avidin is used in conjunction with polyethylene glycol to coprecipitate biotin-labeled carcinoembryonic antigen (CEA) antibody and the CEA-antibody complex together with biotinlabeled normal serum proteins. The procedure provides a reliable separation technique for a CEA radioimmunoassay. The technique is broadly applicable to assays employing immune precipitation.