Autotrophic growth of hydrogen bacteria in continuous culture

✍ Scribed by Dr. J. V. Voytovich; I. I. Gitelson; P. I. Ponomaryev; F. Ya. Sidko; I. A. Terskov; I. N. Trubachov

• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 269 KB
• Volume: 12
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19720120111

✦ Synopsis

A laboratory installation was developed for continuous cultivation of hydrogen bacteria by turbidistate with a capacity of about 5 1. A steady state process with Hydrogenomom eutropha was realized which produces up to 40 g/l . day of dry biomass. Optimum concentration values for oxygen, hydrogen, carbon dioxide in the gas phase for various densities of bacterial suspensions were found. The minimum concentrations of bioelements are : N 50-70 mg/l, P, Mg, K 20-40 mg/l.

The biomass of hydrogen bacteria is characterized by a high protein content (up t o 77%) including irreplaceable amino acids up to 40%, and nucleic acids up t o 10%. Oxygen and nitrogen deficiency lead t o a protein decrease up to 46% and an increase in the concentration of poly-B-hydroxybutyric acid. The possibility of a continuous unsterile cultivation of Hydrogenomonus with prevalence over the growth of the accompanying microflora is shown.