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Autoradiographic distribution of μ-, δ- and κ1-opioid stimulated [35S]guanylyl-5′-O-(γ-thio)-triphosphate binding in human frontal cortex and cerebellum

✍ Scribed by Stefan Platzer; Anett Winkler; Jan Schadrack; Dominik Dworzak; Thomas R Tölle; Walter Zieglgänsberger; Rainer Spanagel

Book ID: 117470611
Publisher: Elsevier Science
Year: 2000
Tongue: English
Weight: 555 KB
Volume: 283
Category: Article
ISSN: 0304-3940
DOI: 10.1016/s0304-3940(00)00943-5

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An in vitro autoradiographic technique has recently been developed to visualize receptor-activated G-proteins by using agonist-stimulated [35S]guanylyl-5'-O-(gamma-thio)-triphosphate ([35S]GTPgammaS) binding in the presence of excess guanosine 5'-diphosphate. This technique was used to localize opio