Automation of micro-preparation and enzymatic cleavage of gel electrophoretically separated proteins
β Scribed by Tony Houthaeve; Heinrich Gausepohl; Matthias Mann; Keith Ashman
- Book ID
- 115931574
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 308 KB
- Volume
- 376
- Category
- Article
- ISSN
- 0014-5793
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π SIMILAR VOLUMES
A simple custom-made gel holder for preparative SDS-PAGE to separate aggregated bacterial antigens is described. The gel holder fits easily into commercially available gel electrophoresis apparatus and proteins or peptides are collected in a stream of distilled water through a channel in the gel. Th
## Abstract A microchip gel electrophoresis (MCGE) method with electrokinetic supercharging (EKS, electrokinetic injection with transient isotachophoresis) on a single channel chipΒ was developed for highβsensitive detection of a standard mixture of six proteins (phosphorylase __b__, albumin, ovalbu