Automation of immunochemical analysis—A prototype instrument to determine diuron or atrazine in water
✍ Scribed by Petra M. Krämer
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 249 KB
- Volume
- 9
- Category
- Article
- ISSN
- 0895-7533
No coin nor oath required. For personal study only.
✦ Synopsis
A prototype system of an automated, computercontrolled immunochemical analysis for pesticide residues in water will be described. This system is based on flow-injection analysis. The immunochemical reaction, which is the fundamental and specific part of this technique, is carried out in an immunoaffinity column with protein A on a polymetacrylate surface. Antipesticide antibodies, pesticide standard or water sample, enzyme tracer, and substrate for the enzyme reaction are pumped sequentially into this column. The measurement of one sample currently needs 1 hour and 45 minutes, which includes the preceding measurement of a control value. The fluorescence of the product of the enzyme reaction is expressed as percentage of control (zero standard, set as 100%). Within a European demonstration project (program Life), measurements are currently carried out for phenylurea and s-triazine herbicides (diuron and atrazine) in order to show the suitability of this technique as an on-line monitoring system and as an automated laboratory instrument. Standard curves for diuron and atrazine showed ranges of detection from 0.02 to 0.5 lg/L. For a simulation of real water samples, mixtures of diuron with four different concentrations of monuron, atrazine, and isoproturon, respectively, were measured. Nearly all mixture combinations showed a decrease