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Automatic testing of enzyme modifiers by the flow-gradient technique

✍ Scribed by Juliana Marcos; Angel Ríos; Miguel Valcárcel

Publisher
Elsevier Science
Year
1995
Tongue
English
Weight
580 KB
Volume
308
Category
Article
ISSN
0003-2670

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✦ Synopsis

The potential of the flow-rate gradient technique for the automatic testing of enzyme modifiers is demonstrated by using the acetylthiocholine/acetylcholinesterase system. Modifier concentration gradients are created in the enzyme flow by establishing flow-rate gradients via computer control of the peristaltic pump used to propel the modifier solution stream. A single solution of each modifier and a single experiment is sufficient to obtain information about the modifier effect and to quantify the modifier/substrate ratio above which it is detected. 33 substances were tested and their effects on the enzyme system quantified by spectrophotometric detection. The procedure provides a rapid, simple means for studying modifiers affecting any enzyme system.

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