Automatic synthesis of phosphopeptides by phosphorylation on the solid phase
β Scribed by H.B.A. de Bont; J.H. van Boom; R.M.J. Liskamp
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- French
- Weight
- 280 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
The seven phosphopeptide derivatives based on the native -NEYTA- sequence of the pp60src protein kinase family, Asn-Glu-Tyr(P)-Ser-Ala, Ala-Glu-Tyr(P)-Ser-Ala, Ala-Ser-Tyr(P)-Ser-Ala, Ala-Ser(P)-Tyr-Ser-Ala, Ala-Thr-Tyr(P)-Ser-Ala, Ala-Thr(P)-Tyr-Ser-Ala and Ala-Ser(P)-Tyr(P)-Ser-Ala, were prepared
A new procedure for the phosphorylation and assay of phosphoproteins is described. Proteins are solubilized from tissue samples, separated by polyacrylamide gel electrophoresis, transferred onto nitrocellulose membrane filters, and the blotted polypeptides are phosphorylated with the catalytic subun