Automatic sugar analysis in the beet industry. Part I: Parameter optimization of a reversed phase HPLC carbohydrate determination
✍ Scribed by Herbreteau, Bernard ;Lafosse, Michel ;Morin-Allory, Luc ;Dreux, Michel
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 441 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0935-6304
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✦ Synopsis
Abstract
An evaluation of the most commonly used HPLC system (reversed phase octadecyl sillica gel) was undertaken in order to determine the level of certain carbohydrates in molasses produced in the refining of sugar beet. Chromatographic parameters and purification operations prior to analysis are discussed in order to develop an analytical method permitting automation of sugar determination. A Zorbax ODS column (250 × 9.4 mm), water elution, and light scattering detection allow easy determination of glucose + fructose, sucrose, and raffinose in molasses using an internal standard (maltose).