Automatic sample preparation of sulfonamide antibiotic residues in chicken breast muscle by using dynamic microwave-assisted extraction coupled with solid-phase extraction
✍ Scribed by Hui Wang; Yang Xu; Weitao Song; Qi Zhao; Xiaopan Zhang; Qinglei Zeng; Haiyan Chen; Lan Ding; Nanqi Ren
- Publisher
- John Wiley and Sons
- Year
- 2011
- Tongue
- English
- Weight
- 318 KB
- Volume
- 34
- Category
- Article
- ISSN
- 1615-9306
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✦ Synopsis
Abstract
In the work, a rapid, simple and high‐throughput sample preparation method was developed for the determination of sulfonamide (SA) antibiotic residues in chicken breast muscle. The extraction and clean‐up were online combined and up to 20 samples can be treated simultaneously in 6 min. The SAs were first extracted with acetonitrile under the action of microwave energy, and then the extract was directly introduced into the SPE column for on‐line clean‐up and concentration. Subsequently, the SAs eluted from the SPE column were determined by liquid chromatography–tandem mass spectrometry. The precisions of extraction results of 20 samples were in the range of 4.9–7.4%. The limits of detection and quantification obtained were in the range of 2.4–3.6 ng/g and 8.6–11.3 ng/g for SAs, respectively. The recoveries of SAs obtained by analyzing chicken muscles at three fortified levels (10, 50 and 500 ng/g) were in the range of 82.6–93.2%. The results of the validation process prove that the proposed method is suitable for treating numbers of complex samples simultaneously in a short time.