Automatic apparatus for heterogeneous enzyme immunoassays based on electrocatalytic detection of the enzyme and electrochemical regeneration of the solid phase

✍ Scribed by Denis Huet; Christian Bourdillon

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 794 KB
• Volume: 272
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)80570-b

✦ Synopsis

A glassy carbon electrode, anodized for a short tune at about 2 2 V vs SCE, IS a very convenient regenerable solid phase for the suCCesswe stages of an automated enzyme-linked immunosorbent assay The anodized surface adsorbs the pnmary antibody and the blockmg protems m a snmlar manner to class& polystyrene solid phases, the detection of the munobdlzed glucose oxldase used as the label can be obtamed from an electrocatalytlc process occurring directly on the electrode, and the protein stackmg, accumulated dunng the assay, can be ehmmated by a simple re-anodlzatlon at the end of the cycle, allowmg the adsorption of a new pnmary antlbody An automatic apparatus has been developed round an electrochemical flow cell fed with samples and reagents accordmg to the pnnclples of flow-mJectlon analysis Using rabbrt IgG as analyte and glucose ondase as label, senes of 150 successwe assays were conducted on the same electrode before It was polished again The overall time of one assay, including the time for pnmary antibody adsorption and electrode regeneration, was 55 mm