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Automatic and Quantitative Measurement of Protein-Protein Colocalization in Live Cells

✍ Scribed by Costes, Sylvain V.; Daelemans, Dirk; Cho, Edward H.; Dobbin, Zachary; Pavlakis, George; Lockett, Stephen


Book ID
119988807
Publisher
Biophysical Society
Year
2004
Tongue
English
Weight
519 KB
Volume
86
Category
Article
ISSN
0006-3495

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We describe the quantitative monitoring of TATA-binding protein (TBP) localization and expression in living Saccharomyces cerevisiae cells. We replaced the endogenous TBP with a green fluorescent protein (GFP) β€’ TBP fusion, which was imaged quantitatively by laser scanning confocal microscopy (LSCM)