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Automated Ultra-Thin-Layer SDS Gel Electrophoresis of Proteins Using Noncovalent Fluorescent Labeling

✍ Scribed by Csapo, Zsolt; Gerstner, Arpad; Sasvari-Szekely, Maria; Guttman, Andras


Book ID
125985193
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
100 KB
Volume
72
Category
Article
ISSN
0003-2700

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A new method for visualizing 2D protein spot patterns is described whereby proteins containing sulfhydryl groups are labeled with the fluorophore monobromobimane prior to the isoelectric focusing step of 2D polyacrylamide gel electrophoresis. The method requires the addition of a single reagent and