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Automated sample processing using robotics for genetic typing of short tandem repeat polymorphisms by capillary electrophoresis

✍ Scribed by Phillip Belgrader; Michael A. Marino

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 120 KB
Volume: 9
Category: Article
ISSN: 0895-7533
DOI: 10.1002/(sici)1098-2728(1997)9:1<3::aid-lra2>3.0.co;2-4

No coin nor oath required. For personal study only.

✦ Synopsis

We have assembled a robotic system that couples DNA purification from bloodstain cards with polymerase chain reaction (PCR) amplification in an automated and uninterrupted process. This system will enable thousands of samples to be processed a day and is designed to be modular to allow peripheral devices to be added or upgraded as needed. Current work involves using the robotic system to produce PCR amplification products derived from loci consisting of short tandem repeats (STRs). To further reduce labor hours, these products are then detected by automated capillary electrophoresis, which eliminates the requirement to cast gels and manually load samples.

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