Automated rapid method for microbioassay of amino acids

✍ Scribed by Hiroshi Itoh; Tomoaki Morimoto; Ichiro Chibata

• Book ID: 102625621
• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 428 KB
• Volume: 60
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(74)90269-3

✦ Synopsis

iln automated rapid method for microbioassay of amino acids was investigated by taking advantages of the rapid manual method. The Pye Unicam automatic analytical apparatus was adopted for the automation of assay culture in the rapid microbioassay. The advantages of the present method are that amino acids can be automatically determined on 3.5-hr assay culture, and that an aseptic technique can be omitted. These advantages were confirmed in several amino acid assays. The assay values were the same as those obtained by the conventional method. Application of the automated rapid method to the serine assay showed a linear standard curve without a lag section, leading to more expanded assay range and smaller drift in valves.