Automated proton titrations of pancreatic phospholipase A2

✍ Scribed by Gabriëlle M.Donné-Op den Kelder; Gerard H. de Haas; Maarten R. Egmond

• Book ID: 102985838
• Publisher: Elsevier Science
• Year: 1982
• Tongue: English
• Weight: 584 KB
• Volume: 125
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(82)90396-7

✦ Synopsis

A computer-controlled titration system has been developed, tested, and used for analysis of the proton-titration behavior of pancreatic phospholipase A2 from ox, horse, and pig. The results revealed an error in the reported amino acid composition of the equine enzyme. A simple interface for the input of digitized data from a pH meter is described together with the software developed for controlling the titration experiments.