✍ Scribed by Stefanie Terstegge; Iris Laufenberg; Jörg Pochert; Sabine Schenk; Joseph Itskovitz-Eldor; Elmar Endl; Oliver Brüstle
No coin nor oath required. For personal study only.
Embryonic stem cell (ESC) technology provides attractive perspectives for generating unlimited numbers of somatic cells for disease modeling and compound screening. A key prerequisite for these industrial applications are standardized and automated systems suitable for stem cell processing. Here we demonstrate that mouse and human ESC propagated by automated culture maintain their mean specific growth rates, their capacity for multi‐germlayer differentiation, and the expression of the pluripotency‐associated markers SSEA‐1/Oct‐4 and Tra‐1‐60/Tra‐1‐81/Oct‐4, respectively. The feasibility of ESC culture automation may greatly facilitate the use of this versatile cell source for a variety of biomedical applications. Biotechnol. Bioeng. 2007;96: 195–201. © 2006 Wiley Periodicals, Inc.
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