Automated lanes detection and comparison of bacterial electrophoretic protein fingerprints using fast Fourier transformation
✍ Scribed by Sally Millership; K. Ragoonaden
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 1006 KB
- Volume
- 25
- Category
- Article
- ISSN
- 0010-4809
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✦ Synopsis
A method of computer-automated analysis of bacterial fingerprints produced by electrophoresis of proteins in a one-dimensional slab gel system is described. Proteins were visualized by silver staining. Western blotting, or autoradiography. Gels were recorded with a CCD camera, and after initial manual removal of the unwanted image margins, track margins were identified and extracted and a normalized trace was produced automatically using Fourier routines to smooth plots required for this process. Normalized traces were then compared by Fourier correlation after application of a high-pass step filter.