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Automated Identification of Amino Acid Sequence Variations in Proteins by HPLC/Microspray Tandem Mass Spectrometry

✍ Scribed by Gatlin, Christine L.; Eng, Jimmy K.; Cross, Stacy T.; Detter, James C.; Yates, John R.


Book ID
121296555
Publisher
American Chemical Society
Year
2000
Tongue
English
Weight
205 KB
Volume
72
Category
Article
ISSN
0003-2700

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The determination of the primary structure of peptides and proteins is routine in many laboratories; however, many of the obtained sequences are incomplete or can be misinterpreted when the samples contain unusual amino acids. Here we report the development of an automated peptide sequenator coupled