Automated flow-injection serial dynamic dialysis technique in the study of drug binding with cyclodextrins

✍ Scribed by E.E. Sideris; C.A. Georgiou; M.A. Koupparis; P.E. Macheras

• Book ID: 102987025
• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 788 KB
• Volume: 289
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(94)80011-1

✦ Synopsis

AbStNCt

A flow-injection dynamic dialysis technique is presented for the determination of binding parameters of drugs to cyclodextrins (CDs). The automated system consists of a flow-injection unit, the sample loop of which is the receiving compartment of a dialyser unit, and a home made timing module for operation control through two flow switching solenoid valves. The procedure of binding studies is rapid and yields reproducible results. Binding parameters of CD-micromolecule complexes were calculated using the Scatchard model. Apical examples of the binding of p-nitrophenol with (r-CD (K, = 1.58 X lo3 M-' at pH 7.4 and 2.06 X lo3 M-r at pH 9.01, salicylic acid with @CD (K, = 3.8 x 10' M-' at pH 1.5 and 51 M-r at pH 7.4) and ibuprofen with &CD (K, = 2.2 x lo2 M-' at pH 2.5) are presented and the binding constants obtained are compared to literature values. 1: 1 stoichiometry was found in all cases and within run precision ranged from 2 to 14% R.S.D. The between run precision for the binding of p-nitrophenol to a-CD was 2% (n = 3).