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Automated DNA extraction from genetically modified maize using aminosilane-modified bacterial magnetic particles

โœ Scribed by Hiroyuki Ota; Tae-Kyu Lim; Tsuyoshi Tanaka; Tomoko Yoshino; Manabu Harada; Tadashi Matsunaga

Book ID
118471629
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
385 KB
Volume
125
Category
Article
ISSN
0168-1656

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Bacterial magnetic particles (BMPs) were modified with 3-[2-(2-aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 ร‚ 10 4 surface amines were detected on a