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Automated determination of enzymatic rate functions by the ‘gradient-flow’ method

✍ Scribed by Patricia A. Gurr; J. Tze-Fei Wong; Charles S. Hanes

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
291 KB
Volume
51
Category
Article
ISSN
0003-2697

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✦ Synopsis

Under optimal experimental conditions a gradient-flow method has been employed t,o obtain an automated recording of the velocity-substrate relationship for the enzyme horse liver alcohol dehydrogenase. Distortions due to mechanical factors as well as due to excessive reaction could be eliminated. A general criterion for safeguarding the validity of rate functions recorded by this method has been proposed.

PRINCIPLE OF METHOD

The progress curves of most enzymic reactions typically have a slope (i.e., velocity) which falls off with time. The amount of product P, formed after a fixed reaction time t will be a useful measure of the initial velocity 584

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