Automated determination of DNA using the fluorochrome Hoechst 33258

✍ Scribed by W. Sterzel; P. Bedford; G. Eisenbrand

• Book ID: 102629789
• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 394 KB
• Volume: 147
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90299-4

✦ Synopsis

An automated method for the determination of DNA content in fractions from the alkaline filter elution assay of DNA damage has been developed. DNA-containing fractions are mixed with a tluorochrome (Hoechst 33258) and the DNA concentration is measured fluorometrically in a continuous-tlow system. The lower limit of detection is 0.05 pg DNA/ml, and the linearity range under the conditions used is O-8 Gg DNA/ml. The standard deviation (n = 10) was found to be +0.83%. The results are compared with the manual method. 0 1985 Academic PRS, Inc.