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Automated analysis of human sperm motility

✍ Scribed by D. Schoëvaërt-Brossault

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
690 KB
Volume
17
Category
Article
ISSN
0010-4809

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✦ Synopsis

Sperm motility is a determinant parameter characterizing the fertilizing ability of a semen sample. An objective method for measuring this parameter based upon an automated analysis of microcinematographic recordings is presented. The system, which consists of a Cinescan H16, a Quantimet 720 image analyzer, and a PDP 11134 minicomputer. allows a motility analysis to be performed in about 5 min.

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Seminal plasma constituents were separated on Sephadex G200 gel columns. The column eluate was analysed with regard to protein content, ATPase activity and promotive activity on sperm progressive motility. Two different chromatographic fractions were also subjected to electron microscopy after sedim