Autolysis of Thermoactinomyces vulgaris spores lacking carbon dioxide during germination
✍ Scribed by Dr. S. Kretschmer; H.-E. Jacob
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 545 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0233-111X
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✦ Synopsis
Ultrathin sections of early germinating endospores of !Phermoactinomyces vulgaris were studied by electron microscope. Only spores aerated with a n air-COB mixture (5% C02) grow out, while spores aerated with air (0.03% CO,) lyse by the 25th min of inoculation. The lysis is due to progressive, unlimited degradation of the spore integuments and a lack of cell wall formation around the spore protoplast.
The requirement of CO, for outgrowth could not be replaced by oxaloacetate. CO, seems to be needed to energize the dormant cytoplasmic membrane of the spore t o render i t capable of initiating active transport processes and of synthesizing the germ cell wall.