Autohydrolysis of Phytic Acid

✍ Scribed by Steven R. Hull; James S.S. Gray; Rex Montgomery

• Book ID: 102559876
• Publisher: Elsevier Science
• Year: 1999
• Tongue: English
• Weight: 119 KB
• Volume: 273
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1999.4220

✦ Synopsis

The autohydrolysis of phytic acid at 120°C resulted in the formation of most of the phosphate esters of myo-inositol in varying amounts depending upon the reaction time. Eighteen of the 39 chromatographically distinct myo-inositol mono-, bis-, tris-, tetrakis-, pentakis-, and hexakisphosphates have been characterized using two different HPLC systems. These myoinositol phosphates were partially purified by preparative anion-exchange chromatography under acidic and alkaline elution conditions. The combination of these two methods provides a two-tiered chromatographic approach to the rapid and sensitive identification of inositol phosphates in complex mixtures. Identification of the products was confirmed by 1D and 2D 1 H NMR analysis. The analytical procedure was applied to the autohydrolysis of the mixture of inositol phosphates from corn steep water.