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Autofluorescence removal, multiplexing, and automated analysis methods for in-vivo fluorescence imaging

โœ Scribed by Mansfield, James R.; Gossage, Kirk W.; Hoyt, Clifford C.; Levenson, Richard M.


Book ID
120451156
Publisher
The International Society for Optical Engineering
Year
2005
Tongue
English
Weight
343 KB
Volume
10
Category
Article
ISSN
1083-3668

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## Abstract Standard fluorescence microscopy approaches rely on measurements at single excitation and emission bands to identify specific fluorophores and the setting of thresholds to quantify fluorophore intensity. This is often insufficient to reliably resolve and quantify fluorescent labels in t