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Autofluorescence methods in ophthalmology

✍ Scribed by Rovati, Luigi; Docchio, Franco


Book ID
111875069
Publisher
The International Society for Optical Engineering
Year
2004
Tongue
English
Weight
372 KB
Volume
9
Category
Article
ISSN
1083-3668

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Cellular autofluorescence affects the sensitivity of flow cytometric assays by interfering with detection of low level specific fluorescence. These detection limits increase with use of protocols, such as thermocycling and fluorescent in-situ hybridization (FISH), that can increase intrinsic cellula