Autocrine TGF-β-regulated expression of adhesion receptors and integrin-linked kinase in HT-144 melanoma cells correlates with their metastatic phenotype
✍ Scribed by Bassam Janji; Chantal Melchior; Valérie Gouon; Laurent Vallar; Nelly Kieffer
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- French
- Weight
- 276 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0020-7136
No coin nor oath required. For personal study only.
✦ Synopsis
We have previously shown that 2 human melanoma cell lines, the metastatic HT-144 and the non-metastatic SK-Mel-2 cells, exhibit marked in vitro heterogeneity with respect to integrin expression, migration and invasion potential. Here, we provide evidence that HT-144 melanoma cells, but not SK-Mel-2 cells, undergo a reversible transition to a fibroblastoid morphology following treatment with either their own serum-free acidified conditioned medium or biologically active exogenous TGF-1, thus identifying TGF- as an autocrine regulator of the spindle shape morphology of HT-144 melanoma cells. The fibroblastoid phenotype correlated with up-regulated 1 and 3 integrin and downregulated E-cadherin expression, as shown by flow cytometry, Western blot and RT-PCR, as well as up-regulated expression of the matrix metalloproteinase MMP-9, as demonstrated by zymography. Our data further illustrate the TGF-1-dependent up-regulation of integrin-linked kinase and the nuclear translocation of -catenin, 2 intracellular proteins involved in integrin and cadherin signaling.