Autoantigenic epitopes of the b polypeptide of SM small nuclear RNP particles

✍ Scribed by Yasuo Ohosone; Tsuneyo Mimori; Takao Fujii; Masashi Akizuki; Yasuo Matsuoka; Shoichiro Irimajiri; John A. Hardin; Joe Craft; Mitsuo Homma

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 700 KB
• Volume: 35
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780350818

✦ Synopsis

Objective. To analyze the autoantigenic epitopes of the Sm B polypeptide of the U1 small nuclear RNP (snRNP) using complementary DNA (cDNA) clones.

Methods. Expression of Sm B fusion proteins in A phage vectors, immunoblots, immunoprecipitations, and a h i t y purification of antibodies.

Results. Immunoblots using antibodies affinitypurified from B fusion proteins demonstrated that there were cross-reactive epitopes between the B'/B and A polypeptides of the U1 snRNP. Immunoprecipitation