AUTHOR'S REPLY.

✍ Scribed by COOPER, K.

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: English
• Weight: 115 KB
• Volume: 179
• Category: Article
• ISSN: 0022-3417
• DOI: 10.1002/(sici)1096-9896(199605)179:1<125::aid-path550>3.0.co;2-p

✦ Synopsis

Having recently made the same error, I wish to draw your attention to the paper by Wright et al. entitled 'Detection of herpes simplex virus DNA in spontaneous abortions from HIV-positive women using non-isotopic in situ hybridization'.' I believe that these cases are not of herpes infection; what the authors have observed are the biotin-containing intranuclear inclusions that are not uncommonly present in gestational tissues. This phenomenon has recently been described by Yokohama et aL2 and Sickel and di Sant'Agne~e.~ Since these inclusions contain biotin, immunohistochemistry and in situ hybridization using biotinylated signals will lead to false staining.