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Auramine O as a fluorescent probe for the binding of basic drugs to human α1-acid glycoprotein (α1-AG) The development of a simple fluorometric method for the determination of α1-AG in human serum

✍ Scribed by Yuichi Sugiyama; Yasuyuki Suzuki; Yasufumi Sawada; Seiji Kawasaki; Tomoe Beppu; Tatsuji Iga; Manabu Hanano


Book ID
115778636
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
757 KB
Volume
34
Category
Article
ISSN
0006-2952

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A novel probe was developed to measure drug association with the F1\*S variant of the human serum protein alpha 1-acid glycoprotein (AGP). The molecule 2hydroxy-3,5-diiodo-N-[2(diethylamino)ethyl]benzamide (DEDIC) binds to AGP, quenching its native ¯uorescence. This quenching was ®tted to a two-site